brdu primary antibody  (Cell Signaling Technology Inc)

Journal: Materials Today Bio

Article Title: Carrier-free supramolecular architectonics of quercetin – berberine for safety-redefined therapy of ulcerative colitis

doi: 10.1016/j.mtbio.2026.102970

Figure Lengend Snippet: QB enhances epithelial barrier integrity and promotes colonic mucosal repair. A) Immunohistochemical staining of tight junction proteins Occludin and ZO-1 in colonic tissues (scale bar: 150 μm). B) Immunofluorescence staining of BrdU and β-catenin in colonic tissues (scale bar: 150 μm).

Article Snippet: Multiplex TSA-Based Immunofluorescence Staining: Dual immunofluorescence staining was performed on paraffin-embedded sections using primary antibodies against BrdU (Servicebio, GB12051, 1:200) and β-catenin (Proteintech, 51067-2-AP, 1:200), followed by HRP-conjugated secondary antibodies (Pinuofei Biotech) and TSA amplification with TYR-488 and TYR-555.

Techniques: Immunohistochemical staining, Staining, Immunofluorescence

Journal: Stem Cell Reports

Article Title: Microbiota from young mice restore the function of aged ISCs

doi: 10.1016/j.stemcr.2025.102788

Figure Lengend Snippet: Elevated levels of Akkermansia muciniphila in aged animals cause attenuation of both Ascl2 -based canonical Wnt signaling and ISC function (A) Venn analysis of the difference between the microbial taxa in the indicated FMT groups. “ ∗ ” represents the groups in which microbial species or families enriched in young or aged animals after FMT. (B) Frequency of reads of A. muciniphila upon FMT. (C) Relative level of expression of Ascl2 , Lgr5 , and β-catenin in crypts of aged mice treated with A. muciniphila or vehicle. (D) Relative level of expression of Ascl2 , Lgr5 , and β - catenin in crypts of young mice treated with A. muciniphila or vehicle. (E) Representative pictures of stainings for BrdU lineage tracing in crypts of young and aged and young and aged AB-treated mice. (F) Average distance traveled (from crypt base into the villus) by BrdU + cells in crypts of young and aged and young and aged AB-treated mice. (G) Representative pictures of stainings for BrdU lineage tracing in young and aged mice treated with A. muciniphila or PBS after antibiotic treatment. (H) Average distance traveled (from crypt base into the villus) by BrdU + cells in young and aged mice treated with A. muciniphila or PBS after antibiotic treatment. Note: young and aged AB (antibiotic)-treated mice in E and 4F and G and 4H young PBS and aged PBS after antibiotic treatment, treated with PBS are same. p values are determined by an unpaired t test with Welch’s correction. ∗ p < 0.05, ∗∗ p < 0.005, ∗∗∗ p < 0.0005. n = 3–4 mice/group. Error bars indicate Standard deviation.

Article Snippet: 6-μm-thick paraffin-embedded tissue sections were deparaffinized; rehydrated; permeabilized by heating in 10 mM sodium citrate buffer; stained with BrdU primary antibody (Cell signaling, 1:100 dilution in PBS), Ki67 (Thermo Fisher, 1:100 dilution), pH3 (Cell Signaling, 1:100 dilution), LYSOZYME (Dako 1:400 dilution), and OLFM4 (1:100 dilution); incubated overnight at 4°C; and incubated with anti-mouse FITC-conjugated secondary antibody (Jackson ImmunoResearch Laboratories, 1:200 dilution (both for BrdU and Olfm4 stainings) for 1 h at room temperature or 1:200 anti-mouse biotinylated antibody followed by ABC kit (Vector Shields) or anti-rabbit Cy3-conjugated secondary antibody (Jackson ImmunoReseach Laboratories, 1:200 [for pH3 and lysozyme stainings]).

Techniques: Expressing, Standard Deviation